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Journal / Developmental Biology / Generazione ed espansione di cardiomiociti umani…
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JoVE Journal Developmental Biology
Generazione ed espansione di cardiomiociti umani da cellule mononucleate del sangue periferico del paziente
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Generazione ed espansione di cardiomiociti umani da cellule mononucleate del sangue periferico del paziente

Article DOI: 10.3791/62206-v • 05:38 min • February 12th, 2021
February 12th, 2021 •
1,2, 3, 1,2, 1,2,4, 1,2,5, 3, 1,2,5,6
1Center for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children’s Hospital, 2The Heart Center, Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology, The Ohio State University College of Medicine, 4Department of Anatomy, The Ohio State University College of Medicine, 5MCDB Graduate Program, The Ohio State University, 6Department of Pediatrics, The Ohio State University College of Medicine

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Qui, presentiamo un protocollo per generare ed espandere in modo robusto i cardiomiociti umani dalle cellule mononucleate del sangue periferico del paziente.

Tags

Biologia dello sviluppo Numero 168 cellule mononucleate del sangue periferico PBMC cellule staminali pluripotenti indotte umane iPSC differenziazione cardiaca riprogrammazione iPSC espansione cardiomiocitaria.
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