Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

October 27th, 2023

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03:39 min

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Preparation of Plate for Fluorescent Imaging and Cell Viability Assessment in CAR-Jurkat Co-Culture with CFSE-Labeled Tumor Cells (Video) | JoVE

04:00 min

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Siddharth Subham*^1,2,3, John D. Jeppson*¹, Benjamin K. Gibbs⁴, Jacqueline Babai², Riza Alker¹, Andrew K. Godwin^4,5,6, David Akhavan^1,2,3

¹Department of Radiation Oncology, University of Kansas Cancer Center, ²Department of Cancer Biology, University of Kansas Cancer Center, ³BioEngineering Program, University of Kansas, ⁴Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, ⁵University of Kansas Cancer Center, ⁶Kansas Institute for Precision Medicine, University of Kansas Medical Center

* These authors contributed equally

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Tek tümör antijenini hedefleyen kimerik antijen reseptörü (CAR) eksprese eden Jurkat hücreleri tarafından kantitatif tümör hücresi öldürmesini değerlendirmek için bir protokol. Bu protokol, periferik kandan türetilen T hücrelerinde onaylanmadan önce CAR menteşe yapılarının hızlı optimizasyonu için bir tarama platformu olarak kullanılabilir.

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Evaluating Quantitative Tumor Cell Killing by CAR-Expressing Jurkat Cells

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