Universidad de Buenos Aires, CONICET 2 articles published in JoVE Developmental Biology Early Unguided Human Brain Organoid Neurovascular Niche Modeling into the Permissive Chick Embryo Chorioallantoic Membrane Luciano Fiore1,2, Jan Arderiu3, Andrea Martí-Sarrias3,4, Isabel Turpín3,4, Ruth I. Pareja3,5, Arcadi Navarro5,6,7,8, Mariana Holubiec2,9, Julieta Bianchelli9, Tomas Falzone2,9, Gonzalo Spelzini1,2, Gabriel Scicolone1,2, Sandra Acosta3,4 1Instituto de Biología Celular y Neurociencias “Prof. E. De Robertis” (IBCN), CONICET - Universidad de Buenos Aires, 2Facultad de Medicina, Departamento de Biología Celular, Histología, Embriología y Genética, Universidad de Buenos Aires, 3Institute of Neurosciences, Pathology and Experimental Therapeutics Dept, University of Barcelona, 4Functional Neurogenomics Group, Neurodevelopmental Disorders, IDIBELL, L’Hospitalet de Llobregat, 5IBE, Institute of Evolutionary Biology (UPF-CSIC), Department of Medicine and Life Sciences, Universitat Pompeu Fabra, 6Institució Catalana de Recerca i Estudis Avançats (ICREA) and Universitat Pompeu Fabra, 7Center for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, 8BarcelonaBeta Brain Research Center, Pasqual Maragall Foundation, 9Instituto de Investigación en Biomedicina (IBioBA) – CONICET – Instituto Partner de la Sociedad Max Planck Here, we present a protocol to engraft human brain organoids at multiple maturation stages into the chick chorioallantoic membrane (CAM). Brain organoids were grown following unguided standardized protocols. Biology Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence Felipe J. Renna1, Malena Herrera Lopez1, Maria Manifava2, Nicholas T. Ktistakis2, Maria I. Vaccaro1 1Instituto de Bioquimica y Medicina Molecular Prof Alberto Boveris (IBIMOL), Universidad de Buenos Aires, CONICET, 2Signalling Programme, The Babraham Institute The goal of this protocol is to determine autophagic levels in pancreatic cancer and pancreatic acinar cells through LC3 immunofluorescence and LC3 dot quantification.
