Canadian Institute for Advanced Research 4 articles published in JoVE Immunology and Infection T4 Bacteriophage and E. coli Interaction in the Murine Intestine: A Prototypical Model for Studying Host-Bacteriophage Dynamics In Vivo Nicola Pett1, Michael Hunter1, Natalia A. Carranza García1, Jung Hee Seo1, Samuel R. Collins1, Forest Rohwer2, Lisa C. Osborne1, Carolina Tropini1,3,4 1Department of Microbiology and Immunology, Life Sciences Institute, University of British Columbia, 2Department of Biology, San Diego State University, 3School of Biomedical Engineering, University of British Columbia, 4Humans and the Microbiome Program, Canadian Institute for Advanced Research Bacteriophages (phages), viruses that infect bacteria, are an integral component of the gut microbiome. Though these symbiotic inhabitants drive bacterial fitness and population dynamics, little is understood about how they impact gut homeostasis and disease. This protocol studies isolated T4 phages within a mouse model, adaptable to other phage-bacterial pairs. Chemistry Hydrogen Production and Utilization in a Membrane Reactor Alexandra R. Rousseau*1, Mia D. Stankovic*2, Curtis P. Berlinguette1,2,3,4 1Department of Chemical and Biological Engineering, The University of British Columbia, 2Department of Chemistry, The University of British Columbia, 3Stewart Blusson Quantum Matter Institute, The University of British Columbia, 4Canadian Institute for Advanced Research (CIFAR) Membrane reactors enable hydrogenation in ambient conditions without direct H2 input. We can track the hydrogen production and utilization in these systems using atmospheric mass spectrometry (atm-MS) and gas chromatography mass spectrometry (GC-MS). Immunology and Infection Visualization of Gut Microbiota-host Interactions via Fluorescence In Situ Hybridization, Lectin Staining, and Imaging Katharine M. Ng1,2, Carolina Tropini1,2,3 1School of Biomedical Engineering, University of British Columbia, 2Department of Microbiology and Immunology, University of British Columbia, 3Humans and the Microbiome Program, Canadian Institute for Advanced Research (CIFAR) This streamlined protocol details a workflow to detect and image bacteria in complex tissue samples, from fixing the tissue to staining microbes with fluorescent in situ hybridization. Neuroscience Foraging Path-length Protocol for Drosophila melanogaster Larvae Ina Anreiter1, Oscar E. Vasquez1, Aaron M. Allen2, Marla B. Sokolowski1,3 1Department of Ecology and Evolutionary Biology, University of Toronto, 2Department of Cell and Systems Biology, University of Toronto, 3Child and Brain Development Program, Canadian Institute for Advanced Research We provide a detailed protocol for a Drosophila melanogaster foraging path-length assay. We discuss the preparation and handling of test animals, how to perform the assay and analyze the data.
